<%@ Register TagPrefix="eo" Namespace="EO.Web" Assembly="EO.Web" %>
<eo:CallbackPanel ID="CallbackPanel1" runat="server"  Width="100%" AutoDisableContents="True"  LoadingHTML='Please wait...'
                        Triggers="{ControlID:Button1;Parameter:}">

<asp:DataGrid ID="DataGrid1" runat="server" >

 <asp:TemplateColumn HeaderText="Upload" HeaderStyle-Width="4%">
     <ItemTemplate> <asp:FileUpload ID="UploadAttachment" runat="server" EnableViewState="true" /></ItemTemplate>
</asp:TemplateColumn>

</asp:DataGrid>

</eo:CallbackPanel>

Private Sub Upload_Click(ByVal sender As System.Object, ByVal e As System.EventArgs) Handles Button9.Click
        closeitems()
End Sub
Function closeitems() As String
 For i = 0 To DataGrid1.Items.Count- 1
      Dim fileUpload As FileUpload
      fileUpload = CType(DataGrid1.Items(i).FindControl("UploadAttachment"), FileUpload)
      
      ' -*************  PROBLEM COMES HERE ***********-
      If fileUpload.HasFile Then
           Dim filename As String = fileUpload.PostedFile.FileName
           fileUpload.SaveAs(MapPath("~/Upload/") & filename)
           Cmd.CommandText = String.Format("INSERT INTO TABLENAME VALUES({0}, '{1}', {2}, '{3}')", ItemNoValue, filename, DateTime.Now.ToString, Session("UserID"))
           Cmd.CommandType = CommandType.Text
           Cmd.ExecuteNonQuery()
       End If
       Conn.Close()
 Next I
End Function